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Contaminating RBCs were lysed using RBC-lysis solution.
Contaminating RBCs were lysed with ACK lysis buffer (150 mM NH4Cl, 1 mM KHCO3 and 0.1 EDTA pH 7.3).
Cell supernatants were centrifuged at 700× g, and a hypotonic shock of 30 s was applied to cell pellets to remove contaminating RBCs; more than 98% of the cells were PMNs.
The pellet was then suspended in red blood cell (RBC) lysis buffer (Biowhittaker, Walkersville, MD, USA) and incubated for 10 minutes at room temperature to lyse the contaminating RBCs.
The pellet was then suspended in red blood cell (RBC) lysis buffer (Biowhittaker, Walkersville, MD, USA) and incubated for 10 min at room temperature to lyse the contaminating RBCs.
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Of the 8982 stringent nRBC DM sites, six were located in hemoglobin genes we found to be highly expressed in cord blood WBCs sorted by a standard protocol (and thus presumed to be contaminated with RBCs) (Fig. 2; Additional file 1: Table S2).
One remote but distinct possibility is the presence of contaminating red blood cells (RBCs) in the samples of both groups.
Contaminating red blood cells were lyzed using RBC-lysis solution (eBioscience, San Diego, CA, USA).
Single cell suspensions of solid tumours were prepared and contaminating red blood cells removed using an RBC lysis buffer.
Fresh blood was centrifuged at 250 g for 20 minutes, the buffy coat was transferred to a new tube and contaminating red blood cells were lysed with 10 ml of RBC Lysis Solution (5 Prime).
Avoid contaminating your food.
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