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Molecular analyses (LO Hand/or P53 sequencing) confirmed that the population of cells with non-neural phenotype consisted entirely of glioblastoma cells and were not contaminated with normal cells as LOH, without contamination with the normal allele, nor were P53 mutations identified (Fig. 2c,d).
In some instances the complexity of these changes makes them difficult to interpret, particularly when tumour samples are contaminated with normal (stromal) tissue.
By combining with a novel sample mixing model, we demonstrate that MixHMM can also detect the CNV states of tumor cells in a heterogeneous sample contaminated with normal cells (i.e. in a biopsy sample).
Moreover, exfoliated cells are frequently contaminated with normal cells, bacteria, and cellular debris.
In contrast to the cancer tissues that contained normal stromal cells, the 15 cancer cell lines were not contaminated with normal cells.
However, because tumor samples are often contaminated with normal cells, both peaks are typically shifted to the left, on average around 0.7 and 0.35.
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Given that tumors are typically highly heterogeneous and that epithelial tumor tissues are often contaminated with surrounding normal stromal/mesenchymal cells, do the above variations reflect this heterogeneity?
Last Thursday, the company said the groundwater near the plant was contaminated with 10,000 times the normal level of radiation, only to then question its own finding.
However, current methods for determining the breakpoint are either of low resolution or require the isolation of relatively pure cancer cells, which can be difficult for clinical samples that are typically contaminated with various amounts of normal host cells.
The ability to distinguish between normal and tumor DNA is low, particularly because clinical samples, including plasma, mouth washes, or Papanicolaou smears, are routinely contaminated with DNA from several normal cell types.
Considering that plasma DNA samples can be easily contaminated with DNA originating from normal peripheral blood cells, we first analyzed the methylation status of the seven genes in peripheral blood cell samples from four noncancer patients.
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