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ACCNS and CAC2 cells were contaminated with cells derived from non-human mammalian species: the cells labeled ACCNS were mouse cells and the CAC2 cells were rat cells.
Upon analysis of primary A. odorata cultures, we found that they were contaminated with cells of Trichoplusia ni origin.
Leucocyte subpopulations in the wound secretion were recorded as percentage as they were heavily contaminated with cells from connective and other tissues.
We confirmed through RT-PCR that cell cultures were not contaminated with cells of mouse origin that might share identity in gene probe sequence [ 21].
As a consequence of the very high cell turn-over of the surface epithelia the environment is contaminated with cells from hair, skin, nails, eyebrows, eyelashes, airways, etc.
Following detection of GFRa1, membranes were stripped and probed for β-actin, which served as a loading control and confirmation that the supernatant fraction was not contaminated with cells or cellular debris (lower panel).
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To ensure that exosomes were not contaminated with cell-free palmitate we used exosomes collected 9 h after palmitate removal from the medium EXO-Post Palmm). EXO-Post Palm
At a minimum, the degree to which the targeted population is contaminated with unwanted cells or cell fragments must be known if meaningful interpretations of molecular profiles are to be made.
Because of the rarity of the cells in the LN population the sorted cells were contaminated with other cell types yet many gp120+SIGN-R1+ gp120+SIGN-R1+ visualized.
Molecular analyses (LO Hand/or P53 sequencing) confirmed that the population of cells with non-neural phenotype consisted entirely of glioblastoma cells and were not contaminated with normal cells as LOH, without contamination with the normal allele, nor were P53 mutations identified (Fig. 2c,d).
Together, these data confirmed the identity of these cell lines as XEN cells and demonstrated that they were not contaminated with ES cells or any other pluripotent cell type that can be derived from mouse blastocysts.
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