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After plasma collection, the buffy coat layer was removed to a separate the cryotube and contaminated red blood cells were lysed using osmotic shock in sterile water.
The border of Williamtown's contaminated red zone cuts right through the land.
During heavy rains between 16 and 18 February, people living near the plant's two reject basins reported floods of contaminated red water and mud.
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Importantly, merozoite invasion into contaminating red blood cells from the schizont inoculum (auto-invasion) may confound the analysis.
Elutriated T lymphocytes were purified from contaminating red blood cells by centrifugation on Ficoll (Lymphocyte Separation Medium; Cellgro, Manassas, VA) followed by treatment with ACK lysis buffer (Lonza, Walkersville, MD) to lyse erythrocytes.
For buffy coats, enriched leukocytes were subjected to ficoll purification (Amersham) and centrifugation at 1400 rpm with the brake turned off for 30 minutes to remove contaminating red blood cells and platelets.
The BMC were washed several times with PBS and then contaminating red blood cells were lysed with AKC buffer (0.15 M NH4CL, 10 mM KHCO3 and 0.1 mM EDTA, pH 7.35).
Contaminating red cells were removed by incubation in 0.8% ammonium chloride solution for 10 min.
Contaminating red blood cells were lyzed using RBC-lysis solution (eBioscience, San Diego, CA, USA).
Contaminating red blood cells were lysed by suspending cells in 0.85% NH4 in Tris-HCl buffer.
Contaminating red cells were lysed in 0.8% ammonium chloride solution for 10 min.
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