Rapid identification of mycoplasma contaminated cell cultures and materials enables a faster response time to prevent the spread of the contamination.
The crisis of Misidentified and contaminated cell lines have plagued the biological research community for decades.
All cell lines were routinely tested for mycoplasma contamination using a PCR mycoplasma detection kit (TaKaRa, Biomedical Europe S.A., Genevilliers, France) and contaminated cell cultures were discarded.
Therefore, even very low numbers of contaminating cells will produce significant contamination.
Although many of these contaminating cells may be removed during passaging, such contamination would affect the expansion of MSCs as well as the overall effect of the therapy.
An average 95-fold decrease in leukocyte contamination was seen for samples containing high levels of contaminating cells.
Consequently, it is not always clear whether DFAT cells are derived from dedifferentiated mature (lipid filled) adipocytes or from contaminating cells that reside in an impure culture.
Exclusion of these contaminating cells did not alter the differential sphere forming activity (Figure 5).
The contaminating cells in our cardiomyocyte cultures consisted predominantly of cardiac fibroblasts (>95%, data not shown).
Each 30 µl drop contained only one single fiber devoid of contaminating cells.
