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Jon A. Ward, a real estate lawyer in Garden City, N.Y., said shared driveways can also be found when a large piece of property subdivided by a developer contains "flag lots".
The Rtnl1 exon trap line CPTI-001291 contains FLAG and StrepII tags in addition to Venus YFP (http://flybase.org/reports/FBal0262134.html).org/reports/FBal0262134.html
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The Chronic Condition Warehouse contains flags created using numerous established algorithms applied biannually since 1999 to define 21 chronic diseases [ 16, 23- 27].
Each record contains flags indicating a content-rich or content-poor window and, for those records where one of the thresholds has been crossed, the corresponding sequence.
The BRM fragment was inserted into pENTR-D/TOPO and then subcloned into pGWB511 or pGWB517 containing flag or myc tags56, respectively as described above.
To construct the plasmid containing Flag-tagged full length Usa1 or various deletions, USA1 gene was amplified by PCR from wild type or USA1 deletion strains and incorporated into pRS416Gal1 vector containing Flag epitope at C-terminus.
Full-length human APP, pCEP4-APP695, containing myc-tag and flag-tag, was a generous gift from Dr. G. Multhaup (University of Heidelberg) and pcDNA-APP695 containing flag tag was a generous gift from Dr. T. Mizushima (Kumamoto University).
Protein was isolated from diploid strains containing Flag derivatives of Mec1, Mec1-2369G, Mec1-W2368andand Mec1-Δ1.
The remaining lysate was split equally between the purification columns, one containing FLAG agarose and the other containing FLAG agarose pre-incubated with FLAG peptide to prevent FLAG-conjugated proteins from binding to the resin.
RNA immunoprecipitations were performed on HEK-293 or HEK-293 cells transfected with vectors containing FLAG tagged-AUF1 isoforms.
CY5639 and CY5640 containing Flag - tra1 -R3590I and Flag - tra1 -3590I, F3744A were also generated after integration into CY4398 and sporulation.
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