We envisioned chimeric MAP kinase sensors that contain a docking motif and CSox-containing phosphorylation site.
When cells containing a docking platform marked by GFP expression are challenged with a plasmid that contains a MTX-resistance marker between two attB sites, in combination with an integrase-expressing plasmid, correctly integrated products should be resistant to MTX and should fail to express GFP; we initially tried to select targeted integration using either or both of these properties.
This strain contains a zeta docking platform forcing the integration of the expression cassette at this locus.
If this is the case, it is probable the N-terminus of MKS1 contains a 'classical' docking domain.
Further, the SMN promoter region contains a CREB docking site and the expression of the two is possibly interdependent (57, 58).
This piggyBac insertion on the X chromosome contains an attP docking site and a lox cassette encompassing the 3xP3-GFP screening marker and additional transgenes.
As shown in table 1, the highly conserved c-terminal domain of AIF proteins contains a putative PP1c docking motif, R/Kx 0.1 V/IxFxxR/KxR/K, that combines the two previously reported canonical PP1c docking motifs required for PP1c binding in Bcl-xL and Bcl-w proteins [6].
Using directed yeast two-hybrid screens with different MKS1 deletion forms, we found that the N-terminal domain, which contains a putative kinase docking domain, interacts with MPK4, whereas WRKY33 was found to interact with a plant-specific VQ motif-containing domain.
The amino acid sequence of mitochondrial transcription factor A (TFAM), contains a potential ERK docking domain (Fig. S6) [33], [33] and Ser 31, Ser 177 and Thr 122 immersed in an ERK phosphorylation motif, suggesting that ERK could bind and activate the transcription factor and thereby regulate mitochondrial gene expression.
Similarly, one out of the two currently listed X chromosome-linked nonsyndromic mixed deafness proteins (POU3F4) also contains a putative PP1 docking motif.
