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Strains were cultured at 37°C in Luria-Bertani broth or agar containing, where appropriate, 100 µg/ml ampicillin for E. coli or 20 µg/ml erythromycin and 10 µg/ml chloramphenicol for S. aureus.
This strain was transformed by standard procedures and cultured at 37 °C in Luria-Bertani broth or agar (17) containing, where appropriate, 100 μg/mL ampicillin.
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I can envision an e-book version of "Globish," too, that contained links, where appropriate, to some of Mr. McCrum's intellectual journalism, so you could make your own sidebars.
"This system is a single, elegant virus that can be injected anywhere in the brain, which makes it technically easier and less likely for moving bells and whistles to break down," he adds, "and their behavioral equipment was cleverly designed to contain magnets where appropriate so that the animals could be freely moving around".
Cells were diluted to a concentration of 1 × 10 cells ml−1 in serum-free medium containing soluble molecules where appropriate.
Single colonies of each E. coli strain were grown on Luria-Bertani (LB) agar containing kanamycin (25 μg/ml) where appropriate.
Cells were resuspended in polysome buffer (120 mM KCl, 2 mM MgCl2, 20 mM Tris pH 7.5, 1 mM DTT) containing either cycloheximide or pactamycin where appropriate.
To facilitate comparison with published findings we did not exclude calls within the telomeric band (but loci containing these calls are flagged where appropriate in subsequent sections of the results).
The proteins were processed into mature forms where appropriate; these contain no signal peptides, propeptides, intein regions, and only consists of protein chains annotated by the 'CHAIN' field of the Swiss-Prot entry.
A blank run was always first performed using the buffer alone (containing the appropriate concentration of ligand where appropriate).
The medium was then replaced with complete RPMI 1640 containing PBMC and anti CD3/CD28 beads where appropriate.
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