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Six of the twelve slide/gasket assemblies were sequentially disassembled into the first staining dish containing Wash Buffer 1. Once disassembled, the slide was quickly transferred into a slide rack in a second staining dish containing Wash Buffer 1.
The slide containing the array was then removed from the MAUI hybridization chamber while immersed in wash buffer I (1X SSC, 0.05% SDS), placed in a slide rack containing wash buffer I and washed twice in the same buffer for 5 min with agitation.
After 15 min binding, the RNA binding beads were retrieved using a magnetic plate and washed first in DNase (ThermoScientific) containing wash buffer and thereafter washed and eluted in the same buffer composition as used for the DNA extraction [ 5].
Slides were disassembled in a dish containing Wash Buffer 1 at 42°C and washed in Wash Buffer 1, Wash Buffer 2, and Wash Buffer 3 for 2 minutes, 1 minute, and 15 seconds respectively.
In brief, 50 µL reactions containing wash buffer II, 10-50 proteinotein, 5000 pmol Ser/Thr phosphopeptide ± 5 µM ABA with 0.005 % EtOH as solvent were incubated for 10 30 min at room temperature.
Then the culture medium containing the labelling solution was removed, cells were washed with serum containing wash medium and fixed with 200 µl of precooled 0.5 M ethanol in HCl for 30 min at −20°C.
Similar(52)
The final pellet containing washed microvesicles was resuspended in HBSS for subsequent analysis.
These cartilage shavings were transferred to a Falcon tube containing washing solution (sterile PBS + 10% Pen/Strep) and were twice washed with rotation for 20 minutes.
The first distribution point for basic family hygiene kits (containing washing necessities such as soap, toothbrushes and toothpaste) was the severely overcrowded Bantul district hospital.
The one on the west contained washing facilities and a gutter leading to a soak-away in the central part of the building, the room to the east may possibly have been a guardroom.
It is at first sight perplexing why haplo-insufficiency mutations would not also cause the disease if WASH complexes containing mutant WASH are non-functional.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com