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pBS1479 TAP-tag containing vector [48] and pMM016b genomic unc-119 containing vector [49] were digested with HindIII and NotI.
In contrast, isogenic mutant 2221ΔPEL containing vector pDC123 failed to show hemolytic activity (Figure 7A).
Control cells were transfected with pCFLAG3-KCR1 alore or cotransfected with HCN2 and the empty FLAG-epitope containing vector.
Parental strain MGAS2221 containing vector pDC123 gave a typical β-hemolytic morphology when streaked onto agar plates containing 5% sheep blood.
For experiments in mammalian cells we used the same 6xHis-Tag containing vector designated CFP-xa-YFP as described before [1].
When we produced the tat/rev shRNA containing vector, RSC-UsI-SC46-IMPGW, using this helper plasmid with the mutated site I rev, the vector titers increased 38-fold (Figure 4B).
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Ready-to-use lentiviral particles containing vector-encoding human MT2A shRNA were purchased from Santa Cruz Biotechnology Santaa Cruz, CA, USA).
Previously, we developed a vaccination regimen that involves priming with recombinant vaccinia virus LC16m8Δ (rm8Δ) strain followed by boosting with a Sendai virus-containing vector.
To explore the role of PKCα in cellular sensitivity to platinum compounds, resistant cells were transfected with a PKCα-containing vector.
We believe our clinically relevant smCBA-containing vector may be well suited for this task.
Briefly, both the env-containing vector and pJR101 were digested with BamHI and NotI restriction enzymes.
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