Sentence examples for containing the tandem from inspiring English sources

For mutagenesis assays, the mutagenised libraries were generated by error-prone PCR using pGBKT7 containing the tandem BRCT domains of MDC1 or MCPH1 as templates.

(a) Expression in yeast of proteins containing the tandem BRCT of MCPH1 or MDC1 fused to the GAL4 DNA-binding domain (DBD), but not to the activation domain (AD) of GAL4, lead to growth inhibition and a small colony phenotype.

To extend this observation, we cloned the final 333 amino acids of BRCA1 containing the tandem BRCT domains into the yeast high copy expression plasmid pDEST52.

Importantly though, only very weak binding was observed between p22phox and a fragment of NOXO1 containing the tandem SH3 domains plus the C-terminal tail, implying that autoinhibitory interactions do indeed exist within NOXO1.

A 1 5 dilution of the ChIPed fragments were used in a PCR reaction to specifically amplify the cassette containing the tandem 12 N sequences.

We managed to improve the performance of Arrays II and III by eliminating most clones containing the tandem repeat sequence using the restriction enzyme SspI, for which there was a recognition site in most of the repeats (data not presented).

Additionally, a 288 bp long rsd-fragment containing the tandem-promoter rsd P1 and P2 was obtained by cleavage of the plasmid pUC18-rsd-up with BssHII and HincII.

The enhancer and its complementary oligo containing the tandem-repeat Runx2 sites (underlined: 5′-GATCCACAATTAGG TGTG GGTGTGGCCAGCA-3′) were synthesized with or without 5′-biotinylateion (IDT Technology, Coralville, IA, USA).

Both full-length RIα and the truncated RIα 92 381) form that contains the tandem CNB A and B domains are rather stable and this thermal stability is enhanced in the presence of cAMP.

We studied the thermal denaturation of full-length RIα and a truncated RIα 92-381) that contains the tandem cyclic nucleotide binding (CNB) domains A and B. As revealed by circular dichroism (CD) and differential scanning calorimetry, both RIα proteins contain significant residual structure in the heat-denatured state.

The truncated monomeric RIα 92 381) protein that contains the tandem CNB A and B domains, but lacks the highly helical N-terminal docking/dimerization (D/D) domain [32], was selected to further investigate determinants for thermal stability of RIα and the apparent structural conversion upon denaturation.