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The strain was kept on agar plates containing the same medium with 15 g/L agar added for solidification.
After 8 days growing in suspension, the cell aggregates were transferred to gelatin-coated dishes containing the same medium to allow the cells to attach.
Cells (2×105/well) in media containing 0.5% serum were added into the inserts and placed in wells containing the same medium plus TGF-β (5 ng/ml) as chemotactic factor.
Spheroplasts from each transformation reaction were mixed with 15 ml melted synthetic complete agarose medium lacking uracil but containing 1 M Sorbitol and 10 µg/ml cycloheximide and poured onto plates containing the same medium.
The longevity of adult flies was recorded using new born flies passed into fresh vials containing the same medium and a drop of live yeast, in an incubator at 25°C with an identical light/dark regime.
After 72 h on P3, populations were transferred to a new plate (P4) containing the same medium with the same concentration of salt (80 g/L).
Similar(46)
The lower well contained the same medium with 10% FBS.
They were transferred to fresh pots containing the same MS medium and incubated in a growth room at 25°C.
Control microcosms were prepared by incubating parallel vials containing the same culturing medium with filter-sterilized groundwater samples.
In our assay, a small aliquot of the pex5 Δ culture grown in a nutrient-rich medium containing 0.5% glucose and recovered from mid-logarithmic phase was transferred into each well of a 96-well master microplate containing the same growth medium and a compound from a commercially available library.
We found that the sensitivity of bloodstream-form T. brucei to compound 1 was indeed higher in a medium with inosine as the only purine source, compared to the same medium containing the same concentration of hypoxanthine (P < 0.02).
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CEO of Professional Science Editing for Scientists @ prosciediting.com