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That target is thought to be a complex containing the protein annexin A2.
Bacterial flagella are helically shaped structures containing the protein flagellin.
Typically, a syringe containing the ligand is titrated into the cells containing the protein solution.
First the researchers bathe donor immune cells in a solution containing the protein.
The IPG strips were rehydrated for 12 h in 250 μL of rehydration buffer containing the protein samples.
After solvent evaporation, a polymer film, containing the protein, is then deposited on the porous scaffold surface.
The fractions containing the protein were collected, pooled, and further purified by ion exchange chromatography using a Resource Q column (GE Healthcare).
As negative controls, we detected the growth of transformants co-transformed with the fusion plasmid containing the protein and the pGADT7 or the pGBKT7 free vector.
pLink (Yang et al. 2012) is the program used for querying a database containing the protein sequence and for identifying the cross-linked peptides.
The smallest network containing the protein hypocretin (HCRT) was also the consensus network for the DR4/DR4 group.
1,8-ANS was added (final concentration 5 µM) to a solution containing the protein or the buffer only (blank).
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