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Data in FCS file format containing the mouse lung epithelial RNA-seq expression from Treutlein et al.23.23
The shRNA sequences were cloned into a version of pBluescript containing the mouse U6 promoter, as described previously14.
A plasmid containing the mouse Smarca2 complementary DNA (cDNA) clone was purchased from Transomic Technologies (clone ID BC075641, IMAGE ID 30544092).
To knockdown CstF-64 and CPSF-73, a cassette containing the mouse U6 promoter and the specific shRNA sequence was excised from pBluescript and subcloned into pLenti6 (Life Technologies), as described previously51.
Fans pull airborne particles into a disposable disc containing the mouse cells, which have been genetically engineered to emit blue light when exposed to one of six agents, including anthrax and smallpox.
MS/MS spectra were searched by the Sequest algorithm against a database containing the mouse DGAT1 protein database and its reversed complement.
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Frozen bacterial stocks were thawed, sonicated, diluted to ∼106 bacteria/ml, and nebulized in an aerosol infection chamber (Glascol) containing the mice.
Nfatc3 transcripts containing the mouse-specific 3′ exon IV were expressed at moderate levels in the thymus and spleen.
The box containing the mice was then placed in the CT gantry for thorax scanning.
In addition, the start byte is now compressed with part of the mouse data; the first half of the start byte is overhead, and the second half contains the mouse button info and sign bits for the movement.
The sand therefore contained the mouse scent signals produced within a 24-hour period, but not bedding odours.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com