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Transgenic diatom clones containing the introduced pPhAP1-EGFP vector did not express EGFP fluorescent at high phosphate concentration, and exhibit growth curves similar to that of wild type strain.
Therefore, we cultivated transgenic rice lines containing the introduced HvNAS1 (gNAS1), HvNAS1, and HvNAAT-A and HvNAAT-B (gNAS1 + gNAAT), or IDS3 (genes) genes (Fig. 2) in a field with Andosol soils, which are highly porous, dark-colored soils developed from parent material of volcanic origin and one of the typical soil groups in Japan.
The cells are then plated on the appropriate medium to select for transformants containing the introduced plasmids (Protocol 2).
Notably, the transformed strains are prototrophic and ostensibly isogenic, besides containing the introduced gene of interest at the manipulated loci, compared to their parental strain.
The expression levels of BCAR3, EGFR and BCAR1 were not strongly affected in the various cultures, except for the cells containing the introduced transgene.
An in-house script that can take an entire genome as input and return a mutated genome with a given error rate and a file containing the introduced mutations with their type (substitution or indel) and their genome position was used.
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It contains the introduced HSUPA related protocol functions, namely TCP/IP, RLC, MAC-d, MAC-e/es and Iub/Iur Framing Protocol for E-DCH.
Re-introduction of L857 back to the S gene of Vero-adapted IBV allowed recovery of variants that contain the introduced L857.
Positive transformants were administered additional rounds of selection in order to obtain homoplasmic cell lines in which all copies of the chloroplast genome contained the introduced chimeric hGAD65 gene.
Furthermore, the H2/CO mixture addition resulted in an increase in CO2 post filter, the levels of which were proportional to the volume of CO contained within the introduced mixture.
All genes were sequenced to ensure that they were in-frame with YFP and contained only the introduced synonymous mutations.
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