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The C28-GUSh fusion protein was expressed by eukaryotic CHO cells containing the expression construct and secreted into the supernatant of these cells.

Briefly, prokaryotic vector E. coli BL21 (DE3) strain containing the expression plasmid was cultured and induced to express HBc VLPs.

Finally BL21 DE3) containing the expression plasmid 2 (with two T7 promoters each at the upstream of rbcL and rbcX) was chosen as the best expression system for Rubisco.

After stable transfection and differentiation in vitro, met-enkephalin and pro-enkephalin cells had higher met-enkephalin immunoreactivity and secreted met-enkephalin levels, compared to control cells containing the expression vector only.

Transformed colonies of host cells containing the expression vectors were grown in 1 liter LB medium containing 100 µg/ml ampicilin at 37°C overnight to logarithmic phase.

The resulting recombination inserted shuttle vector sequences containing the expression cassette and pIX-MT fusion gene into the pAdEasy1 adenovirus backbone replacing the E1A and E1B sequences.

Expression vectors for CFP-ubiquitin and mRFP-PCNA were constructed using a previously described method in which a HindIII SalI NotI three-way ligation into pXPSN results in a SpeI fragment containing the expression cassette [37].

Sf-9 cells were transfected with a Bacmid containing the expression cassette from pFastBac-C-His-strep-AKAP18γ.

Starting from any existing network supplemented with expression data, the user has to select the attribute field containing the expression information.

We applied the PPA to the mouse-human data sets matched through 8,942 one-to-one orthologous genes, containing the expression signal from 27 organs of both species.

A pre-processed series matrix file containing the expression profiles of 91 mouse cell and tissue types was downloaded from the NCBI Gene Expression Omnibus (GSE10246).

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