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According to the results of SDS-PAGE and Western blot analyses, compared with E. coli BL21 DE3) transformants containing pET-29a, E. coli BL21 DE3) transformants containing the expression vector pET-AGAB-4 expressed the rAgaB-4 with the His-tag (attached to the C-terminal end); the molecular weight of the band was similar to the expected molecular weight of 97.32 kDa (Fig. 1).
The expression profiles are standardized on a per-gene basis in each dataset, and a matrix containing the expression profiles of all genes differentially expressed in at least one of the conditions is created.
The C28-GUSh fusion protein was expressed by eukaryotic CHO cells containing the expression construct and secreted into the supernatant of these cells.
Briefly, prokaryotic vector E. coli BL21 (DE3) strain containing the expression plasmid was cultured and induced to express HBc VLPs.
In the control, A. bipunctata larvae were fed with meal moth eggs that were either coated with buffer solution without the Bt toxin or with buffer solution containing the expression vector pBD10 (the 'empty construct' containing all materials expressed by the vector except the target trait, the Bt toxin) to exclude effects of the production method itself.
After the pET-32a-GP73 pET-32a-GP73 pET-32a-GP73nto host E. coli BL21 (DE3), a single clone containing the explasmidn vector was cultransformedA600 = 0.6 and intoced to express thostE.ombinant protein GP73 with 1 mM IPTG at 25°coli
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In the case of a batch reaction it is to contain the expression "batch" instead.
Each cell in this matrix contains the expression level of a gene (row) under an experimental condition (column).
By convention, backout commit messages contain the expression "backout"—or one the variations "back out", "backing out", and "backed out"—, followed by the hash of the commit being backed out.
Typically, the microarray cancer studies provide only a limited number of tissue samples from the specified classes of tumours or patients, whereas each tissue sample may contain the expression levels of thousands of genes.
Both combinations contain the expression cassettes for all seven transgenes (OCT4, SOX2, NANOG, LIN28, c-MYC, KLF4 and SV40LT).
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CEO of Professional Science Editing for Scientists @ prosciediting.com