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In addition, a PyMOL.pse session file showing the regions of interest and an excel file containing the backbone RMSD data computed from the simulated trajectory are made available to download.
The user also gets an excel file containing the backbone RMSD values for K17I and L35P mutants, which can be downloaded and analyzed by the user in order to infer the overall stability of the mutants.
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The site contains the backbone atoms of Met26, Val32, and Val34 and side chain of Lys31 (Figure 5G).
This results in one or more MUL-trees, all of which exhibit the same collection of clusters and that contain the backbone tree as a subtree.
Two donor acceptor (D−A) copolymers, PEHBDT-BTz and PODBDT-BTz, containing the same backbone of benzodithiophene (BDT) and bithiazole (BTz) units but different side chains were designed and synthesized.
The digestion yielded a HF-soluble fraction and the HF-insoluble fraction containing the PGN backbone.
The 6.3-kb DNA segment containing the plasmid backbone was extracted from gel and its 3' recessed ends were filled in with Klenow polymerase (Fermentas).
inoculation of 20 μl of IRIV, or 20 μl IRIV containing the plasmid backbone (pcDNA3).
As a control we transduced cells with a vector containing the same backbone but without the β3 integrin cDNA cassette.
Another vaccine (Suvaxyn PCV2 One Dose, Pfizer Animal Health/Fort Dodge Animal Health) is based on a chimeric PCV1-2 virus containing the genomic backbone of the non-pathogenic PCV1, with the ORF2 capsid gene replaced by that of PCV2 [ 4].
To do this we transduced cells with a bicistronic lentiviral vector that constitutively expresses Sca-1 and neomycin resistance (Fig. 6A), and as a control we also transduced cells with a vector containing the same backbone but without the Sca-1 cDNA cassette.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com