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An equivalent volume of sterile saline was added to microcosms not containing strain CNP-8.
Compared with the empty-vector (pET21a) and predicted protonation-site mutant D407N containing strain, the wild type AcrB constructed in pET21a showed apparent resistance against ciprofloxacin.
The results showed that the transcription level of dnrO and master regulator dnrI, was found to be elevated in bldA containing strain in comparison to dnrO overexpressed strain.
The bldA containing strain produces 45.7% higher DNR than bldA deficient wild type strain from culture broth with OD600 of 1.45 at 72 h.
Moreover, the GDH enzyme activity was evaluated with the strain E. coli BL21 DE3 pLysS and its plasmid containing strain E. coli BL21 DE3 pLysS/dhaD, and was found to be on average 38.9.00-fold increased in E. coli BL21 DE3 pLysS/dhaD, which was directly correlated with an increase in the expression of GDH from plasmid pJET1.2/blunt.
As shown in Table 1, after 30 days of feeding with diet 2 containing strain GFP-2, the salmon showed greatly higher gained weight (3.8 fold vs. 1.1 fold) and higher survival rate (79.0% vs. 29.8%) than those with the control diet.
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PCR products obtained from amplification of chaperonin 60 genes (cpn60; coding for GroEL) were hybridized to a custom-designed microarray containing strain-specific cpn60-based 35-mer oligonucleotide probes.
These observations suggest that phaZ gene is overexpressed in the plasmid containing strains.
The plasticity region of Helicobacter pylori (H. pylori) is a large chromosomal segment containing strain-specific genes.
Consistently, the PTS genes were mostly located inside genomic islands containing strain-specific sets of PTS genes, indicative of high rates of duplications, losses and gains.
This is illustrated by the recent finding that in Helicobacter pylori, plasticity zones, containing strain-specific genes, actually consist of a mosaic of several ICE, Tn and IS elements [ 85].
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