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In addition to the ability to bind the retroviral capsid protein, the retroviral restriction factors Fv1, Trim5α and Trim5 CypA share the common property of containing sequences that promote self-association.
To imitate the synergism of SOD and GPx, a 65-mer peptide (65P), containing sequences that form the domains of the active center of SOD and the catalytic triad of GPx upon the incorporation of some metals, was designed on the basis of native enzyme structural models; 65P was expressed in the cysteine auxotrophic expression system to obtain Se-65P.
We used ELISPOT assays to test the ability of 15-mer peptides containing sequences that fit the motif to stimulate IFN-γ release by PBMC from these three ECs.
These NATs are endogenous RNA molecules containing sequences that are complementary to other transcripts.
All SNP containing sequences that fulfilled these criteria were defined as putative candidate genes.
SSR containing sequences that showed polymorphisms among these genotypes were selected for primer design.
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The best glycine/serine containing sequence, that turned out to be best for CPR display, was then introduced into the linker for displaying CYP1A2.
In nature, antisense RNAs contain sequences that are the complement of the normal coding sequences found in mRNAs (also called sense RNAs).
Kaminski's groundbreaking work with Spielberg contains sequences that will clearly outlive them both -- the opening D-Day segment in "Saving Private Ryan," a hulking dinosaur staring into a child's window in "The Lost World," the decadent sheen of "Schindler's List".
Critical to the success of a panning experiment is the complexity of the library: the greater the diversity of clones within the library, the more likely the library contains sequences that will bind a given target with useful affinity.
An analysis of the consensus family motifs using the PATCH program in the Transfac database indicated that 19 of the 30 consensus motifs contain sequences that correspond to experimentally confirmed binding sites for specific plant TFs (Table 1).
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