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To construct an expression vector the DNA fragment with a sequence identical to immunodominant regions of HSV-2 gG2 was cloned into modified vector pET28a containing of the glutation-S-transferase sequence (pET28-GST).
Patch pipettes of 5 10 MΩ resistance were filled with a solution containing of the following (in mM): 105 K-gluconate, 30 KCl, 10 HEPES, 10 phosphocreatine, 4 ATP, and 0.3 GTP, adjusted to pH 7.35 with KOH.
To investigate the binding of AP-tagged proteins to Lrp4 in a cell-free system, media containing of the Lrp4 ectodomain fused to the constant region of Immunoglobulin G (Lrp4ecto-Fc) was produced by transfection of HEK293A cells with pcDNA3-LRP4ecto-Fc in DMEM plus 0.2% BSA medium.
We used the Dutch translation [ 63] in a computerized version, containing of the following sections: Depression, Mania, Panic Disorder, Social Phobia, Separation Anxiety Disorder, Specific Phobia, Generalized Anxiety Disorder, Obsessive Compulsive Disorder.
The FMDs are superior to cisplatin, which has poor target selectivity and is highly toxic due to the containing of the heavy metal (Pt) readily binding to proteins in kidney (Townsend and Hanigan, 2002, Zhang et al., 2006), though cisplatin has also generally stronger toxicity against cancer cells.
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"All it contained of the brown lives it had eaten; it was a living thing".
The new wave of cases will increase the difficulty of containing the spread of the disease.
Two mobs attacked a van suspected by some of containing members of the Brotherhood.
The warehouse containing copies of the novel was bombed.
The milk is quite nutritious, containing all of the essential amino acids, plus carbohydrates and lipids.
Every year the CFDA releases a journal containing photos of the nominees.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com