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Alignments of SOD3v1 and SOD3v2 with these four previously identified transcripts indicates the transcripts from the large-scale projects are most likely partially spliced mRNAs containing introns or fragments of intronic sequence (results not shown).
Among overexpressed transcripts, inverted repeat containing introns were not significantly enriched, suggesting that intronic RNA structure in tdp-1 mutants results in reduction of mRNA abundance.
The sequences of MSP genes containing introns seem to be more conserved than those without introns.
The identities of deduced amino acid sequences of the MSP genes containing introns were 96.0 100% within and between the two nodule worms, and were 81.1 93.7% compared with other published MSP sequences of the representative nematodes.
The lengths of the MSP sequences containing introns were 433 bp or 439 bp in O. dentatum, and 436 bp, 439 bp or 446 bp in O. quadrispinulatum, containing 1 or 2 introns.
Since genes containing introns were omitted in this first analysis, in the second step we analyzed all the annotated P. falciparum ORFs (Pf annotations) available from PlasmoDB.
It suggests that histone gene containing introns had been duplicated frequently or homologus recombination occurred several times in the Laccaria bicolor genome.
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Our experiments also reveal previously unknown splicing isoforms, Casp-8x and Casp-9x, as a result of skipping of exons upstream of their In100-containing introns (Figure 6).
These ESTs clearly show a gradient distribution, with significantly higher frequency of EST representation of the exons immediate downstream of In100-containing introns.
All NAGNAG-containing introns with supporting EST data for E- and I-acceptor were divided into subsets of 64 motif classes, according to the heptameric motif NAGNAGN.
Cleavage of microRNA-containing introns by Drosha occurs co-transcriptionally (Morlando et al, 2008) as does cleavage of β-globin terminator region RNA (Dye and Proudfoot, 2001).
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