Sentence examples for containing flag from inspiring English sources
pALHDQ32, pALHDQ43 encoding full-length human FLAG-Q32 and -Q43 huntingtins, respectively, were generated in pFASTBAC1, modified to insert a polylinker containing FLAG, 6X histidine tag sequence and TEV protease recognition site, between the unique BamHI– KpnI sites.
To construct the plasmid containing Flag-tagged full length Usa1 or various deletions, USA1 gene was amplified by PCR from wild type or USA1 deletion strains and incorporated into pRS416Gal1 vector containing Flag epitope at C-terminus.
Full-length human APP, pCEP4-APP695, containing myc-tag and flag-tag, was a generous gift from Dr. G. Multhaup (University of Heidelberg) and pcDNA-APP695 containing flag tag was a generous gift from Dr. T. Mizushima (Kumamoto University).
The DNA fragments encoding PB1 C-terminal sub-constructs corresponding to amino acid sequences 586 696, 676 757 and 576 757 (Uniprot: 31341) were amplified by PCR, and cloned in the same way to generate plasmids pYAN011, pYAN012 and pYAN013 containing FLAG tagged PB1 bait proteins.
The bound materials were eluted by a buffer containing FLAG peptide (0.2 mg/ml).
The remaining lysate was split equally between the purification columns, one containing FLAG agarose and the other containing FLAG agarose pre-incubated with FLAG peptide to prevent FLAG-conjugated proteins from binding to the resin.
The proteomic analysis of the p160-containing protein complex also confirmed the physical interaction of p160 and the VHL ubiquitin ligase complex (Figure 3): FLAG-p160 was transfected into 293T cells and the protein complex containing FLAG-p160 was purified by anti-FLAG immunoprecipitation under non-denaturing conditions.
The 369th began as the 15th New York National Guard Infantry Regiment, and decades later, after Mr. Miles had himself joined a National Guard unit in Harlem, he stumbled on a dusty storage room containing flags, helmets photographs and other relics from the 369th.
The activity observed with transfection reactions containing Flag-GFP were set to 100%.
3-µl aliquots of medium containing Flag-His6-tagged NECD were incubated for 2 minutes on 2% CC Affinity Grids.
In addition, we observed in more basal regions internal vesicles containing FLAG-Cold which do not stain positively for Nrg-GFP (Fig. 8D D″).
