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In the synthesis of AuNPs, classical reducers such as mercaptoundecanoic acid [19] and macromolecular containing end amino units such as (i.e. PVP-NH2) [20] are the most important type of stabilizing and reducing molecule to gold nanoparticles of any size.
Further characterization of RIG-I activation requirements showed that dsRNA complementarity of at least 10 18 nt was required at the 5′ppp containing end in order to induce RIG-I activity.
While these types of behavior can be inferred by experiments such as these, the development of methods in vitro to culture mammary explants containing end buds such that extending end buds could be imaged in real time would constitute a major advance.
The SOLiD data, when compared to Illumina's, indicate more dispersed distribution of length, higher frequency variation for nucleotides near the 3'- and 5'-ends, higher frequency occurrence for reads containing end secondary structure (ESS), and higher frequency for reads that do not map to known miRNAs.
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They have been shown to contain predominantly sulfonate and non-sulfur containing end-groups derived principally from initiation by bisulfite radicals and transfer to the bisulfite ion.
They have been shown to contain small quantities of acrylamide and acrylic acid units and to possess predominantly sulfonate and non-sulfur containing end-groups derived principally from transfer to bisulfite ion during the polymerization.
It is performed by transformation with a linear, non-replicative DNA fragment carrying a selectable marker and containing ends homologous to the particular locus in a genome.
The fragmentized cDNAs containing 3' ends were purified from the magnetic beads, and then the Illumina adaptor 1 was added to the 5' ends of these cDNA fragments.
A-tailing was performed in a total reaction volume of 50 μl containing end-repaired DNA, 5 μl 10X buffer, 3 μl Klenow Fragment, 1 μl dATP and 11 μl water at 37°C for 30 minutes.
Subsequently, without any preamplification, the purified DNA fragments were converted into a sequencing library by a one-tube reaction containing end-repair, adenylation, and ligation followed by carrier PCR.
A-tailing was performed in a total reaction volume of 50 μl containing end-repaired DNA, 5 μl 10× buffer, 3 μl Klenow fragment, 1 μl dATP, and 11 μl water, followed by an incubation at 37°C for 30 minutes.
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