We would have also expected to identify deficiencies uncovering zip (in 60E12 on 2R; [24]), and Rho kinase (in 14F2 on X; [26]), but the Exelixis collection did not contain deficiencies for these intervals.
(We estimate that these deficiencies represent roughly 45% genome coverage because the entire Deficiency Kit from the Bloomington Stock Center contains 469 deficiencies, which together provide 98% coverage of the genome).
In contrast, old X chromosomes in Drosophila have become demasculinized, containing a deficiency of genes with male-specific expression [ 27].
To further analyse the relationships between dpn and the members of the E spl) complex, we induced MARCM mutant clones containing the deficiency E spl Xp (Bardin et al., 2010) and simultaneously depleted dpn by means of a dpn RNAi.
During wing disc development, the mutant clone cells, containing a deficiency that eliminates all the members of this complex, did not cause loss of wing margin; however, the differentiation of sensory structures and the pattern of veins was affected (de Celis et al., 1996b).
In a Swiss study, 89 of 264 (34%) discharge prescriptions contained qualitative deficiencies and 72 (27%) showed DRPs.
KEGG XML data files frequently contain minor deficiencies, inconsistent, incomplete or even error records because of manual curation.
However, in three cases, multiple interacting genes were found for one deficiency (two deficiencies contained two interacting genes each, and one deficiency contained three interacting genes), suggesting that the stronger phenotype of the deficiency may arise in part from the reduction of multiple interacting genes.
Finally, to determine whether the ok1251 allele is a null, we performed a deficiency analysis by crossing ok1251 into a strain containing the hDf9 deficiency that removes the kri-1 locus and quantified the number of germ cell corpses after DNA damage.
For the Fe deficiency and excess treatments, four-week-old plants were transferred to nutrient solution containing 0 (Fe deficiency), 100 (Control), or 500 (excess Fe) μM Fe-EDTA and cultivated for one week.
