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In the present study, the in vivo clearance of recombinant rabbit serum albumin (rRSA), of mutant rRSAs containing consensus sequences for N-linked glycosylation (D494N and V14T variants), and of mutant mini-proteins truncated at albumin domain boundaries (rRSAs 1-185, 1-377, or 378-584) was examined.
The target antigen is a branched peptide containing consensus gp41 sequences from multiple HIV-1 subtypes [21].
Deoxyribonucleotides probes containing consensus glucocorticoid response element (GRE): 5-CTAGGCTGTACAGGATGTTCT-GCCTAG-3 and 5-CTAGGCAGAACATCCTGTACAGCCTAG-3 were synthesized and annealed.
Common bioinformatic approaches to identify potential regulatory elements include examining sequences that show significant interspecies conservation, or sequences containing consensus binding site motifs for transcription factors.
Distal p53 binding site was further minimized to 52-mer oligo (probe III) containing consensus p53 binding site and 32-mer (probe IV) deletion mutants, and were used as probe (Figure 4C).
'galign' uses two input files for polymorphism detection: a reference genome sequence file in which all chromosome sequences are concatenated in order, and a sequence-reads file, containing consensus Illumina/Solexa sequence reads on separate lines.
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The fragments contained consensus sequences for each target gene at both ends; therefore, they were inserted into the chromosomal DNA of the host cells through homologous recombination.
Sequence analysis showed conserved five iron binding sites in the LOX domain of all the Rc-LOX, however, only LOX5 contained consensus (positions 547, 556, and 715) histidines residue.
In particular, the SLC6A3 UTRs contain consensus sequences recognizable by eight miBridge' miRNAs, while the SLC6A4 UTRs contain consensuses for 4 miRNA of this class.
In human genomic DNA, only three positions in MKK3 contain consensus sequences for REX1 binding.
It is also straightforward to develop designer vaccines that contain consensus sequences capable of inducing antibody that cross-neutralizes drifted or shifted strains of influenza [1].
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