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For DLS experiments, 400 μL of sample solutions containing assembled nanoparticles (500 nM final) was used.
Contigs were assembled for each EST collection using the program TGICL with default settings (http://compbio.dfci.harvard.edu/tgi/software/) (Quackenbush et al. 2000), and a unigene file containing assembled contigs and singletons was created.
The final number of contigs after merging and splitting was 83,644 (N50 = 1.2 kbp) (see http://genomes.mcdb.ucla.edu/OakTSA/ for files containing assembled contigs, all annotations, variant calls, and other results discussed below).
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Summary: Although de novo assembly graphs contain assembled contigs (nodes), the connections between those contigs (edges) are difficult for users to access.
Many metagenomic repositories contain assembled DNA sequencing reads, which may skew the interpretation of the actual abundance of the organism of interest as opposed to the total number of raw sequence reads.
DBM-DB contains assembled transcriptome datasets from multiple DBM strains and developmental stages, and the annotated genome of P. xylostella (version 2).
In order to further resolve the arrangement of the nine contigs, we made use of the sequence information available from a perennial ryegrass nuclear genome sequencing project that is ongoing in our lab, which contained assembled contigs and scaffolds originating from the mitochondrial genome.
In the early phase of infection, RER of infected neurons showed hypertrophic changes, containing assembling virions within its dilated cisternae.
In order to verify whether this ER staining reflects the presence of E-containing assembled particles, we analyzed the HeLa-prME stable cell line by transmission electron microscopy (TEM) on thin sections of fixed, epon-embedded cells (Figure 3A).
The result of the restriction-ligation is expected to be a 1.17 kb linear fragment containing the assembled GFP exons and introns, in addition to all linear entry vector backbone fragments (2.8 kb).
A compressed file containing all assembled root transcripts and their functional annotations from BLASTX search.
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CEO of Professional Science Editing for Scientists @ prosciediting.com