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A plasmid containing an insert of M. bovis-hspX-M.
Sensitivity was estimated at about 10 molecules per tube of a plasmid containing an insert of the first amplification product.
The vector-harboring clones containing an insert of appropriate sizes (about 1500 bp) were cultured on Luria-Bertani (LB) plates by standard methods (Kaku et al. 2005).
(D) Clones were randomly selected to detect the percentage of clones with a phagemid containing an insert of a proper size for a VHH.
Proteins were produced by Escherichia coli cells transformed with the prokaryotic expression vector pET30a, namely empty vector, or the recombinant vector containing an insert of the X. oryzae hpa1 gene or its truncated version hpa1 30–126 coding for the Hpa110 42 protein.
A commercial plasmid (pReceiver-Z0330-B01, Fulengen, China) containing an insert of the full length open reading frame of the human INA gene was transduced into E. coli BL21-DE3 (Novagen, Sweden), and the expressed recombinant protein was purified by Ni2+ resin (20 mM Tris buffer, pH 10.0) (File S1).
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Transformants that containing a insert of the correct size were then confirmed as correct by restriction enzyme digestion and by DNA sequence analysis.
The clone that included KETc1 was identified by immunoscreening of a cDNA library constructed in UNi-ZAP XR vector, and contained an insert of 1.2 Kb.
For the GR-induced experiment, capped mRNA of a NotI-cut CS2 template contained an insert of the human GR ligand binding domain fused with Nkx2.7.
The CVB4/p24 733) recombinant contains an insert of 243 nucleotides of which 219 nucleotides are from the gag p24 sequence (Fig. 1A).
Several positive clones, which contained an insert of 750 bp were identified and isolated from B. fasciatus venomous gland cDNA library.
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