C. pasteurianum strains were grown anaerobically at 37°C in 2 × YTG medium (16 g l−1 tryptone, 10 g l−1 yeast extract, 5 g l−1 glucose, and 4 g l−1 sodium chloride, pH 6.3) (Sigma-Aldrich; St .Louis, Missouri, United States) within an anaerobic containment chamber (Plas-Labs; Lansing, Michigan) containing an atmosphere of 5% CO2, 10% H2, and 85% N2.
A vehicle is transported from terrestrial point A to B via a closed, ambient-pressure tube containing an atmosphere more aerodynamically favorable than air.
containing an atmosphere of approximately 98% N2 and 2% H2.
After cold incubation, part of the cells were washed with cold HBSS, supplied with cold cell culture medium and rewarmed to 37°C in an incubator containing an atmosphere of 5%CO2/95%% air for 3 h.
We cultured HPDE cells in complete keratinocyte serum-free medium supplemented with 50 μg/mL bovine pituitary extract (BPE), 5 ng/mL epidermal growth factor (EGF), and 1% antibiotic/antimycotic mixture (Gibco/Invitrogen, Rockville, MD) at 37oC in a humidified incubator containing an atmosphere of 5% carbon dioxide/95% air.
Bacteria isolates, previously selected as described above (partially identified as pertaining to Lactobacillus genus), were cultured in de Man, Rogosa and Sharpe broth (MRS, Difco, Detroit, MI, USA) after inoculation with 1% of a fresh stationary culture and incubated in an anaerobic chamber (Forma Scientific Co., OH, USA) containing an atmosphere of 85% N2, 10% H2 and 5% CO2).
And both factors are important for gauging the effects of the solar wind on Earth, Titan, and other solar system bodies that contain an atmosphere of nitrogen.
was used that contained an atmosphere of H2 (3% v/v) and N2 (97%).
Fibroblasts were cultured in an incubator containing a humidified atmosphere containing 5% CO2 at 37°C.
Consequently, packaging of fresh fruits and vegetables contains an ~8% oxygen atmosphere.
The lower atmosphere contains a higher concentration of methane than its upper atmosphere.
