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Cells (2.5 × 10) were incubated overnight in a well of a 24-well plate in 0.5 ml medium containing Abs at a concentration of 10 μg ml−1.
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It was found that the KB-containing EDLCs showed fairly high capacitance (150 210 F/g) compared to EDLCs containing AB with the aqueous electrolyte.
Seeds were inoculated with a commercial product containing Ab-V5 and Ab-V6 strains at concentration of 108 cell ml−1, applied at rate of 100 ml on 60,000 seeds.
The bacterial suspension was washed and resuspended in RPMI (Sigma) containing AB serum (Sigma).
The PSA immunoassay utilized a microreactor to efficiently isolate sandwich immunocomplexes containing Ab-PNIPAAm conjugates within the recirculator via heating.
Prior to co-incubation with infected-monocytes, autologous CD4+ T cells (both Tregs and non-Tregs) were incubated overnight with NAC (10 mM), washed with PBS, resuspended in fresh RPMI containing AB serum (without any stimulants), and then added to the infected monocytes (monocyte: T cell ratio was adjusted to 1 : 1).
As described above, all experiments that involved sorted B cell subpopulations began by removing all non-B cell and plasma cells/plasmablast from PBMCs using a kit that contained Abs specific for CD2, CD3, CD14, CD16, CD36, CD43, CD56, CD66b and glycophorin A, thereby removing plasma cells/plasmablasts that express CD43 and CD66b.
It contains AB, hence AB is automatic.
Even with captain Faf du Plessis suspended for a slow over rate, a batting line-up containing Hashim Amla, AB de Villiers, David Miller, JP Duminy and Albie Morkel might be eying up the likes of Tim Bresnan – seemingly less and less effective with every game he plays and every half-tracker he sends down – and the world's most (fairly, you'd say) maligned bowler Jade Dernbach with some relish.
The vectors expressing the GST fusion proteins containing the AB pockets of Rb, p107, and p130 [43] are a kind gift of Prof. D. Cress.
We incubated HAEC with sera containing HLA-Ab of matching specificities (see Table S1).
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