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A gene, LeCBP encoding a protein containing a PHD zinc finger motif and a bipartite nuclear localization signal, was identified from tomato pistils by its binding ability to a specific DNA motif using a yeast one-hybrid system.
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Five spots at each concentration were then applied to a tile at RT, or one that has been chilled or heated to temperatures ranging from 0 °C to 50 °C, and then sprayed with a mixture containing a pH-sensitive yellow dye, enzyme and substrate.
The optical chemical sensing capability of the RFID tag is demonstrated by measuring pH using a thin sol gel film containing a pH-sensitive indicator dye, bromocresol green (BCG), as a model chemical sensor interface.
Five different spacers containing a pH-sensitive hydrazone bond were synthesized and used to combine a fluorescent model drug with a polymer backbone, conjugated with another non-releasable fluorescent dye.
Herein, we report the fabrication of microparticles composed of two distinct compartments: i) one compartment containing a pH responsive polymer, acetal-modified dextran, and PLGA (polylactide-co-glycolide), and ii) one compartment composed entirely of PLGA.
A study on the controlled release of vitamin B2 in pure water from mesoporous silica-based materials containing a pH- and anion-controlled nano-supramolecular gate-like ensembles built up by anchoring suitable polyamines on the external surface is reported (solid S1).
For the two acids, pH solutions were prepared by titration of the concentrated acid into RO water containing a pH probe under slow stirring until the desired pH value was achieved (within 0.1 standard pH units).
To test this hypothesis, (proteo)liposomes were prepared containing a pH-sensitive fluorophore.
As an indirect means to assess the PIP3 content of the plasma membrane, we examined prepupal leg imaginal discs from flies expressing a fusion protein containing a PH domain linked to green fluorescent protein (tGPH; [56]).
In Isoelectric Focusing (IEF), protein mixtures are electrophoresed in a gel containing a pH gradient.
To more accurately assess the mobility of surface receptors, we analyzed UNC-49B receptors containing a pH-sensitive GFP (pHluorin) tag in the ecto-domain (pH-UNC-49B).
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