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Mutations were detected by differential migration patterns compared to fragments that contained wild type sequences.
However, ten days after induction of clones, no ball mutant FCs were detected (n = 35), but 40% of the germaria (n = 30) contained wild type twin clone FCs and non-mutant control clones were also still present (Fig. 5G,H).
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The irx9 mutant contained wild type-like levels of lignin in the xylem vessels and reduced amounts in the interfascicular fibers.
Crystal violet assay revealed that quite unexpectedly, plasmid cured strain displayed 50% reduction in biofilm forming ability relative to strain containing wild type plasmid (Fig. 3).
Previous study demonstrated that intramuscular injection of plasmid DNA containing wild type Der p 1 gene induced specific Th1 skewed immune response.
Strain Y7029 was transformed with the Not1, Sac1 fragment of pKG15, which contains wild type GAL2.
The pBabe vector containing wild type (WT) HA-Stat1 was described previously [47].
The control Ad5 containing wild type pIX was not recognized by any of these antibodies.
Virions containing mutant L2 proteins were obtained in yields comparable to normal HPV16 virions containing wild type L2.
To construct CEN-proximal cohesion assay strains containing ctf7eco1-1 ADE ctf7eco1-1 ADEining wild typlasmid was geneticontainingneered to contain ctf7eco1-1 DNA that harbors G211D.
B16F10 and MCF-7 cells contain wild type p53, MDA-MB-231 cells contain mutant p53 and PC3 cells are p53 null.
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