Of the 14 informative nonacrocentric SMCs, five (approximately 36%) contained unique DNA from both the p and q arms, whereas nine (approximately 64%) contained unique DNA from only one arm.
The resulting plasmids pBUH3 and pBUH4 contained unique restriction sites of Sac I, Spe I, Bam HI, Sma I, Hin dIII and Kpn I with this order in pBUH3 and with the opposite order in pBUH4 between the maize ubiquitin promoter and a terminator of a nopaline synthase gene.
It included the game, an additional CD, which contained unique armor and weapons and music from the soundtrack, a cloth map, eight character trading cards, and a Black Isle Studios writing tablet.
The PCR primers also contained unique BamHI and KpnI restriction sites, which were used to insert the PCR fragments into pPD95.75.
Subsequent cloning and sequencing revealed that the new strain contained unique capsid gene mutations that contributed to detection failure in the ISH method [52].
The climate data contained unique combinations of latitude and longitude for each species and the corresponding values for the four climate variables.
Prior to mapping, the genetic map was collapsed to retain only markers that contained unique linkage information in the subset of 43 lines (i.e., SNPs at the same map position were removed) leaving 436 biallelic markers.
The resulting recombinant isogenic families (i.e. genetic "clones") contained unique pairs of recombinant autosomes, isolated in males using the attached-autosome balancer T(2∶3 B3 (Bloomington stock 2506) [30].
We prepared four master plates for each target gene: three plates contained unique non-overlapping siRNA duplexes and the fourth plate contained a pool of the three individual duplexes.
Each of the four segments of ∼300 nt contained unique restriction enzyme sites at their termini, which allowed final assembly: fragment 1, 5' NdeI-3' HincII; fragment 2, 5' HincII-3' BsrG; fragment 3, 5' BsrG I 3' BstBI; fragment 4, 5' BstBI 3'XhoI.
Some of our samples contained unique Wolbachia strains.
