Everything is familiar, self-contained, under control: what I need is in easy reach.
The Z/EG transgene contains, under the control of an ubiquitous promoter, a lacZ gene/stop cassette flanked by loxP recombination sites and followed by EGFP [30].
After 3 days, the seedlings were transferred into an aerated hydroponic system containing 0.5 mM CaSO4 under controlled climatic conditions: day/night photoperiod, 16/8 h; light intensity, 220 μmol m−2 s−1; temperature (day/night) 25/20°C; relative humidity 70 to 80%.
Both plasmids contained mtDsRed2 under the control of P1 promoter.
pGmDof4 contained GmDof4 under the control of the Ubiquitin promoter and the selection marker gene nptII.
The third module contained EYFP under the regulatory control of the 3xP3 promoter, and the fourth module contained 671 bp of the 5′ terminal sequences of piggyBac.
However, at this stage most of the cholera cases in the various regions are contained and under control.
In order to improve screening efficiency, we generated stable NCCIT (NCCIT NANOG GFP) cells that contained GFP under control of the human NANOG promoter by lentiviral transduction.
To circumvent this problem, random spore analysis was performed using a query strain of glc7-E101Q that contained GLC7 URA3 under control of the endogenous GLC7 promoter inserted in the ura3 locus (GLC7-res) (see Methods and Additional File 3).
"People should be aware that some of these substances do contain drugs that are controlled under the Misuse of Drugs Act 1971, with more of these drugs being made illegal as they are identified.
For controllable expression of cytoplasmic GFP, a cassette containing gfpmut2 under the control of the T5 promoter, together with a KanR gene, was used to replace the lacZ gene of S. marcescens Db10.
