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The protein crystallized contained two sites of phosphorylation on Thr197 and Ser338.
Site occupancy analyses showed that approximately 48% of N-glycosylated proteins carrying a single N-linked glycosylated site, 20% contained two sites, 16% retained three sites, and the rest carried four or more N-glycosylated sites.
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The final shortlist of five contained four sites in Chile plus La Palma.
The E1 protein contained four sites (positions 196 , 209 234 and 305; numbered as in strain D10988) which are conserved among all variants.
The two genes were FKB12 and TM50a, which contained five and four sites under positive selection respectively.
The 1918 pandemic virus had a hemagglutinin (HA) protein that contained five sites at which post-translational modification by glycosylation could occur [ 27].
Nevertheless, in spite of not being random, this sample contains two sites of a similar type Bitsoup and Filelist and two sites of considerably different audience Etree and Alluvion.
For example, out of 86 annotated coding sequences in the Arabidopsis thaliana chloroplast genome (GenBank NC_000932), only six contain one BsaI site and four contain two sites.
However the intracellular domain also contains two sites for direct binding of TRAF proteins that are conserved among vertebrates [26].
If, on the other hand, the MTase methylates in a processive manner, it is expected that two methyl groups will be incorporated into a DNA molecule containing two sites, resulting in methylation of both sites in single binding event.
Surprisingly, the consensus IGS tandem repeat contains two sites recognized by the FatI restriction enzyme.
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