Sentence examples for contained the selection from inspiring English sources

First, a combined engineering way was used to generate a recombinant strain designated E. coli BL15, which contained the selection of acetoin biosynthetic gene cluster and fine tuning of NADH oxidase expression.

The T-DNA of this vector contained the selection gene bar under control of the Ubi1 intron promoter and the silencing cassette with two restriction sites separated by a rice waxy intron driven by the CaMV 35S promoter and terminated with OCS3' terminator.

In order to delete the remaining Fas2 gene and reuse the disruption construct, the SAT1 flipper cassette containing the selection marker was eliminated in the selected mutant to generate the sensitive heterozygous mutant (Figure 1, lane 3).

Upon selecting a value, a new box containing the selection is sketched, which is connected to the previous box with a line named after the infobox property's key.

Finally, the fourth phase contains the selection of the best allocations' triplet, in terms of maximization of an OF related to utility, i.e., the level of users' satisfaction and the minimization of the associated cost.

The plasmid containing the selection marker module was digested with PmlI, ligated to the plasmid including the integrative module digested with NruI (both enzymes produce blunt ends), and amplified through PCR using the primers YL4-F and YL5-R.

A green fluorescence marker gene eGFP driven by ubiquitin promoter was constructed into pZP200 backbone (http://www.biotech.unl.edu/pzp200) containing the selection marker HptII gene that encodes hygromycin phosphotransferase, was driven by cauliflower mosaic virus (CaMV) 35S promoter (Fig. 2a).

Both of these transposons contain the selection cassette from plasmid pIRES2-EGFP (Clontech), including two promoters, prokaryotic (pbact) and eukaryotic (pSV40), in addition to the Kan/Neo resistance-encoding gene from Tn5 and polyadenylation signals from the HSV TK gene.

Through propagation of the shoots on a medium containing the selection marker, only one line (Atgai-26) was confirmed as transgenic by Southern blot hybridization (Additional file 4).

The incubation-equilibration process is started by gently transferring the 6 (rat) or 10 (mouse) brain slices from the storage beaker (using a micro double-ended spatula) into one 45 mm high, 80 mm diameter, flat-bottomed glass beaker (Duran Group, VWR, Sweden) containing 15 ml (rat) or 10 ml (mouse) of the aECF containing the selection of drugs to be investigated.

Harking back to the Dadaist method of fragmentation, Ms. Rainer chose a collage structure to contain the selections.