The kidneys were then placed in 2 ml tubes and prehybridized in hybridization buffer for 2 hours in a 60°C shaking water bath before being hybridized for 2 nights at 60°C in fresh hybridization buffer containing the DIG-labeled probe.
The membrane was prehybridized in ULTRAhyb hybridization solution (Life Technologies, Carlsbad, CA) for 4 h at 42 °C and then hybridized in ULTRAhyb solution containing the DIG-labeled probe overnight at 42 °C.
Each section was overlaid with 200 μl of pre-hybridization solution [4 × SSC and 50% (v/v) deionized formamide] and incubated at 37°C for 30 min before the solution was replaced with 200 μl of hybridization mix containing the DIG-labeled probe (approximately 20 ng/ slide) and covered with a coverslip.
The Agyptisches Museum contains the treasures dug up by German archaeologists in Egypt, including its most prized piece, the bust of Nefertiti.
After pre-hybridization with hybridization buffer (50% formamide (Sigma), 10% dextran sulfate, 5 mM EDTA, 20 mM Tris pH 8, 10 mM DTT, 1x Denhardt's solution, 0.05% tRNA, 300 mM NaCl) for 1 h at 63°C, the sections were incubated overnight at 63°C with fresh hybridization buffer containing the denatured DIG-labeled sense or antisense probe (200 ng/ml).
The mound containing the archaeologist's dig lies near great fields of bitumen, strange black wastes where fires may leap forth and dance over the ground as freely as the salt springs do.
A cabinet contains the few items that digging had uncovered: a shattered beer stein from the saloon era and cosmetics from a 1920s store.
The barrier allows water to pass through but contains the lead and prevents anyone from digging into contaminated soil underneath.
The membranes were prehybridized for 2 h at 68°C with Roche DIG Easy Hyb and then hybridized overnight at 68°C in DIG Easy Hyb containing the denatured probe.
It also contained a dig at the Scottish leader of the time, Annabel Goldie.
