Sentence examples for contained stably from inspiring English sources
As EGFP is expressed constitutively, this enabled the selection of cells that contained stably integrated viral DNA.
For PRV, 70% of the infected neurons were late viral antigen positive at 3 days post IFNalpha withdrawal (Fig. 2A), often with virus spread to neighbouring non-neuronal cells, indicating that forskolin treatment induced reactivation of PRV in 50% of neurons that contained stably suppressed virus at 5dpi.
The initial test with 199 Ura+ transformants identified eight that were sensitive to 5-FOA, indicating that only about 4% of the transformed cells contained stably integrated DNA.
This result indicates 30% of the transformants contained stably inserted vector DNA, a significant enrichment of mutants with stable integration compared to the previously observed 4%.
A direct comparison of the novel regulatory system with the previously used containing a KRAB-based trans-silencer [ 18] revealed even tighter regulation in Jurkat cells, which contained stably integrated transregulators and which were transiently transfected with a Tet-responsive reporter plasmid (Additional data file 1).
Nanofluid is a new class of heat transfer fluids containing stably suspended nano-sized particles, fibers, or tubes in the conventional heat transfer fluids such as water, ethylene glycol, engine oil, etc. [12 16].
Subsequent treatment of neurons containing stably suppressed virus with forskolin resulted in reactivation of both viruses.
Generation of cell lines often requires the use of cell clones containing stably integrated transgenes.
Therefore, we analyzed whether forskolin was able to reactivate PRV and HSV-1 in the neurons containing stably suppressed virus.
Furthermore, pTRE2hyg-PKCδ-CF-expressing vector and empty vector pTRE2hyg were respectively stably transfected to U937T cells, which contain stably transfected pUHD-tTA (tetracycline responsive transcription activator), whose expression is under the control of tetracycline [27].
Co-infection of human megakaryoblastic leukemia (Mo7e) cells with the rAAV- and rep78-containing hcAd vectors at an MOI of 300 and 800 genomes per cell, respectively, followed by their clonal expansion yielded 25.8% cell clones containing stably transduced Mo7e cells.
