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The mobile phase contained solution A of 0.5 % formic acid and solution B of acetonitrile, with a column temperature of 35 °C and flow rate of 0.3 mL/min.
The medium used for cultivation[ 11] contained Solution A (980 mL) with potassium dihydrogen phosphate (0.4 g), dipotassium hydrogen phosphate (1.2 g), peptone (5 g), yeast extract (1 g), glucose (15 g), final pH 7.2, sterilized at 110 °C in an autoclave; Solution B (10 mL) with magnesium sulphate (0.5 g), filter-sterilized; Solution C (10 mL) with iron II) sulphate (0.3 g), filter sterilized.
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Samples from antrum and corpus were transported in formalin contained solutions and were embedded in paraffin blocks.
The targets contained solutions of indocyanine green (Sigma-Aldrich, St Louis, MO) in concentrations ranging from 1 nM to 1 µM.
The pH of pepsin containing solution was adjusted to 2.0 and trypsin and amylase containing solution was adjusted to 7.5.
Potassium iodate (KIO3) was used as an chemical agent to treat the fluoride containing solution.
Microarc oxidation coatings were fabricated on Ti6Al4V alloy in NaAlO2 containing solution.
The powder containing solution was then put into a Teflon-sealed mini-autoclave (80ϕ × 120 mm).
Three different PN solutions were used: a soya oil containing solution [long chain fatty acids LCT ], an olive oil containing solution, and a solution containing middle and long chain fatty acids MCT/LCT).
The changes in a film formed in an F− containing solution and then placed in a Cl− containing solution are followed by the impedance.
The total RNA containing solution then underwent purification using glass fiber cartridges.
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