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Successful recombinants contained serial loxP sites with one containing the lox66 and lox71 inverted repeat mutants and another with the wild-type inverted repeats.
Then we replaced the medium with another 100 μL of growth medium that contained serial diluents of our compounds and then incubated the cells at 37 °C and 5% CO2 for an additional 72 h.
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All cultures were serially transferred in media containing serial concentrations of kanamycin (15 μg/ml, 100 μg/ml, 200 μg/ml, and 300 μg/ml; X μg/ml kanamycin will be designated as KanX).
The Sydney Literary News (1837) was the first to contain serial fiction and advertisements.
Newspaper supplements, which were particularly influential in the early to mid-20th century, contain serial fiction and short articles on various aspects of daily life in the city.
The eighth column contains serial numbers of the micrographs.
A full spectrum containing serial and parallel resonance frequencies, with a band width of 20 kHz can be scanned within 200 ms at a resolution of 0.2 Hz.
Briefly, suspensions of S. pneumoniae R6 cells (5 × 105 cells/mL) in TH broth containing serial dilutions of the Natto peptide and serial dilutions of the supplements were cultured at 37 °C for 24 h.
The cells in each well were incubated with 100-μL complete medium containing serial concentrations of Au NR@SiO2 nanoparticles (0~36 culture. After 24 h of incubation, 10 μL sterile CCK-8 per well was added and continued to incubate for 2 h at 37 °C (5%% CO2).
3D printed guns also don't need to contain serial numbers — nor do owners need to undergo background checks — which makes them largely untraceable if they're used to commit a crime.
The Huffington Post Kia Makarechi described "Go Missin'" as a "standard track", and wrote that its lyrics contain "serial killer-esque" undertones in the line: Conscience telling you you shouldn't go, don't listen / I'mma take care of you for sure / Follow me right out that back door / Go missin.
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CEO of Professional Science Editing for Scientists @ prosciediting.com