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The latter two sequences were of higher coverage than the genomes overall and assembled into single, circularly permutated contigs that contained read pairs that mapped to the two ends, suggesting that they represent an actively replicating phage.
If the set of reads overlapping X includes a read that is a proper substring of some other read it is possible that the first right terminal extension found is not that of an irreducible edge but of the contained read.
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Each read class contained reads with a fixed number of introduced random errors relative to the consensus genome sequence.
Reads from both libraries were simultaneously detected in 117,533 contigs, whereas 18,908 contigs contained reads from ecodB only and 17,342 contigs contained reads from swB only.
Datasets with longer reads were 3′ trimmed such that all datasets contained reads of equal length.
The first set contained reads mapping to just one genome ("unique reads").
We found that 56,672 contigs (62.42%) contained reads from all six tissues.
First, CABOG incorporates into unitigs the contained reads, according to their best overlaps.
Then, we screened the ACE file for contigs that contained reads in both orientations.
In total, 27 UGTs were selected and contained reads that varied from 4 to 100 bases.
Among contigs, 1,998 contained reads from more than one cDNA insert.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com