Untreated control: One input reservoir contained flow-through medium (RPMI 1640+10% FBS).
The solution was passed through a DEAE cellulose column and the PAP-containing flow-through fraction was then applied to a cation exchange resin S-Sepharose column.
The procollagen-containing flow-through was collected and immediately dialysed against several changes of Q Sepharose buffer (37 mM Tris HCl, 1 mM EDTA, 1 M Urea, pH 8.5 at 4 °C).
This approach allowed us to significantly increase the yield of SWCNTs as compared to the previously employed inert hydrogen-containing flow through the wire.
The two input reservoir contained either flow-through medium or flow-through medium with 200 ng/ml (554 nM) cortisol.
The two input reservoirs contained either flow-through medium or flow-through medium with 361 ng/ml (1 µM) cortisol.
The fractions contained the flow-through proteins eluted with pH 7, 5, 4, and 3, and an organic buffer.
In our previous study, HeLa S100 was separated by Q-Sepharose column into Q-A containing the flow-through and Q-B containing proteins eluted with 0.3 M NaCl (Zeng et al., 2009).
Results: Ga-68-radiolabeling of PSMA-HBED-CC (ABX, GMP grade) was performed using an Advion NanoTek LF system containing a flow-through microreactor that consists of a silica capillary (l = 2 m, Ø100 μm, V = 15.6 μL).
The aim of this study was to test the feasibility of using a fully automated microfluidic system containing a flow-through reactor for the radiosynthesis of Ga-68-PSMA-HBED-CC.
Methods: Ga-68-radiolabeling of PSMA-HBED-CC (ABX, GMP grade) was performed using an Advion NanoTek LF system containing a flow-through microreactor that consists of a silica capillary (l = 2 m, Ø100 μm, V = 15.6 μL).
