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The medium, which contained floating cells, was exchanged for fresh medium at Day 3. At 10 days of culture, adherent cells with a spindle-like morphology were counted under an inverted microscope.
After 48 h treatment, culture supernatants which contained floating dying and apoptotic cells were collected, and then merged with the adherent cells which were rinsed with phosphate buffered saline (PBS) and harvested by a brief trypsinization.
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Floating aquatic plant systems usually contain floating macrophytes.
In this setting, severe inflammation commonly triggers perforation of the gallbladder, namely, abdominal contamination of the bile that potentially contains floating cancer cells; consequently, seeding metastasis often develops.
As patients with biliary tract cancers contain floating cancer cells in the bile [12], bile spillage potentially leads to the development of peritoneal dissemination [13].
With its principal office located in Romney, West Virginia, the South Branch Boom and Lumber Company constructed a log boom on the South Branch Potomac River to collect and contain floating logs timbered from nearby forests.
They ranged from 0.07% (for a ganglioglioma culture containing floating spheres that did not self-renew) to 86% (for an oligoastrocytoma III culture) (Figure S4).
Culture medium containing floating cells was removed and retained.
Medium containing floating cells was gently removed and cells were observed using fluorescence microscopy (Olympus).
Lakes contain floating meadow vegetation, a dense and structurally complex matrix of grass and macrophytes providing habitat cover for fish [ 17].
Do not use water that contains floating material, has an odor or is dark in color.
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