We confirmed that GFP− cells contained expansions and GFP+ cells harboured contractions by sorting cells exposed to both ZFN arms.
Because both polyleucine and polyglutamine containing expansion proteins have been reported to form intranuclear inclusions that are recognized by the 1C2 monoclonal antibody1,21, we performed immunohistochemistry on our mice using the 1C2 antibody and antibody to ubiquitin (Fig. 4).
The results, including abutment displacements, moments at abutment piles, earth pressures on abutments and wingwall, and bridge superstructure forces, are reported and compared with the responses of conventional curved bridges containing expansion joints.
The dataset contains expansion factors (weights) to allow for extrapolation to the U.S. dairy population of the states where the survey was conducted.
To validate this hypothesis, we employed a strategy of transient regulation of apoptosis by using the cell permeable inhibitors of two major apoptotic proteases; caspase and calpian as supplements to the cytokine containing expansion medium.
We established seven lines containing expansions (BAC-Exp) and three control lines (BAC-Ctl).
Arabidopsis and rice both contain expansions in the group III WRKY subfamily [ 29, 73], whereas an expansion of group IIe occurs in potato and tomato [ 72] (Table 3).
The genome of the protist parasite Entamoeba histolytica also contains expansions of tRNAs for which no correlation between copy number and codon usage could be made (Loftus et al. 2005).
While the Obama administration has said it has contained the expansion of the Islamic State in Iraq and Syria, local forces have been unable to dislodge militants from important cities.
The new plan contained "an expansion of high-risk insurance pools, promotion of health savings accounts, and inducements for small businesses to purchase coverage together," as well as "guaranteed renewability" and "the ability to buy insurance across state lines".
It suggests that the Burkoholderia common ancestor probably already contained an expansion of BY-kinases, which was followed by recent duplications in some strains.
