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Of major importance, patches contained defined drug loadings per unit area; therefore, their use could reduce inter-clinician variability.
A series of dilutions were prepared that contained defined numbers of standard molecules, and 5 µl aliquots were used as templates for Real Time PCR.
Cell migration in multi-layer cultures that contained defined 3D distribution of cells is more physiologically relevant than that in conventional migration assays in which cells invade "blank" hydrogel (e.g. Transwell™ migration) or 2D areas free of cells (wound-healing assay).
Twenty of these regions contained defined genes, including one already associated with breast cancer risk: TOX3.
In these experiments, we compared the intensity of western bands in native samples and samples that contained defined amounts of recombinant proteins (Additional file 1: Figure S3).
As the selected oligosaccharide fractions were derived from natural products, the fractions contained defined amounts of mono- and disaccharides and minor amounts of endotoxin, which was taken into account in the design of the study and the analysis of data.
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Sentences containing defined terms are subject to the same logical laws as sentences of the ground language.
Thereafter, 0.1 mL of diluted culture broth (10−6) containing sample B was spread on a plate containing defined medium and 1% skimmed milk.
After incubating 24 h, the media was replaced with fresh BFM containing defined dilutions of antimicrobial compounds for 3 h before the biofilm metabolic activity was measured.
For planktonic assays, cells from the overnight culture were inoculated into THB containing defined dilutions of antimicrobial compounds (i.e., CPC, CPB, CPI).
Antimicrobial activity during biofilm formation was assessed by inoculating cells from the overnight stock cultures into BFM containing defined dilutions of antimicrobial compounds.
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