Total leukocyte counts; lymphocyte, platelet, reticulocyte, and erythrocyte counts; hemoglobin levels; hematocrit values; mean cell volumes; mean corpuscular volumes, and mean corpuscular hemoglobin concentrations were determined from blood containing EDTA by using the HemaVet 950FS Analyzer (Drew Scientific, Dallas, TX, USA).
We tested the mad2 alleles for their ability to arrest cells containing kinetochore defects, by using the temperature-sensitive nuf2-3 atlele at 32°C where ∼30% of cells arrest with short spindles after 4 hr.
Structures were selected considering the nonredundant ensemble (sequence identity lower than 40%) of proteins containing transmembrane regions by using the website PDBTM [ 29].
The immunoprecipitated DNA was amplified for sequences containing binding sites by using the following ABCB1 promoter sequence primers: ABCB1p (F) 5′-CAACTCGTCAAAGGAATTAT-3′ and ABCB1p (R) 5′-TTGTACCTTTGATCAACACC-3′.
After 12 h of an experiment, samples were taken from each tube, serially diluted in PSS, and 100 μL was inoculated onto petri dishes containing nutrient agar by using the spread plate method.
KAAS annotated a total of 746 UTs with KO terms, and these were further classified into 40 pathways containing 382 UTs by using the pathway identification tool in KOBAS.
RNA was extracted from 140 μL of viral transport medium containing the swabs by using the QIAamp Viral RNA Isolation Kit (QIAGEN, Valencia, CA, USA) and amplified by using the Access RT-PCR System (Promega, Madison, WI, USA); primers were directed to influenza A and influenza B viruses according to the standard operating procedure of CDC's Influenza Branch.
We tested for enrichment of GO BP categories amongst the genes contained in the DMRs by using the R package topGO 2.16.0 (Alexa et al., 2006), using as a background set all genes in which it is theoretically possible to detect DMRs.
Nanowires of conducting polymers were directly synthesized through a three-step electrochemical deposition procedure in an aniline-containing electrolyte solution, by using the glassy carbon electrode (GCE) as the working electrode.
Viral RNA was extracted from virus-containing allantoic fluid by using the MagMax-96 AI/ND RNA extraction kit (Applied Biosystems/Ambion, Austin, TX, USA).
