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For each contig, we compared the largest ORF from each of these types and kept the ORF that contained a start codon unless the type B ORF extended upstream of the type A ORF to the beginning of the contig representing cases in which the true start codon is likely truncated from the contig.
A gene was considered as 'complete' if its annotated coding sequence (CDS) contained a start and stop codon.
Moreover, ORFpredictor predicted a higher percentage of start codons in annotated sequences when compared to unannotated: as much as 84% of all contigs contained a start codon.
For example, our original network contained a start node for a small hobby-based club with only a peripheral interest in environmental issues.
The 987 transcripts were translated using the getorf program of the European Molecular Biology Open Software Suite setting a lower limit of 25 aa and including only ORFs that contained a start and stop codon [ 57].
This process identified an additional 23,800 contigs from Kaspa (Additional file 8A) and 28,047 from Parafield (Additional file 8B) which contained a START and STOP codon with minimum sequence length of 100 bp.
Similar(53)
If only it contained a start-over kit.
As mentioned earlier, the execution flow can be represented with an ordered list of intervals, where each interval contains a start time, end time, a state, and the TID of the thread executing for the said interval.
Meeuwisse et al. later recognised that a linear approach containing a start and an end point does not reflect the true onset of injury in sport and proposed a recursive cycle where repeated participation occurs in the absence of injury (Meeuwisse et al. 2007).
A set of 222 non-redundant nucleotide sequences, containing a start and a stop codon, obey the above criteria.
9,901 transcripts did not contain a start codon and 7,778 did not contain a stop codon.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com