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Mixture acting as the microbial inoculum contained a final population density of 1.33 × 108, 2.08 × 108, 2.66 × 108 cfu g−1 inoculum (wet weight) for K, P and N fixing bacteria, respectively.
Reactions contained a final concentration of 0.8 µM of each primer, 1 unit of Taq polymerase and 5 µl of Q solution (Qiagen).
For all PCR reactions in this study, reaction tubes contained a final volume of 25 µL containing 1 ng/µL each of forward and reverse primers, 1 x concentration of GoTaq® Green Master Mix (Promega) and cDNA.
Control wells for forskolin and SKF-83822 contained a final concentration of 0.01% DMSO, although this did not change eGFP expression and control values were collapsed into a single group for analysis.
To investigate the capture of OVA-liposome conjugates by macrophages, macrophage clone 39 was incubated for 30 min at 4°C or 37°C in the presence of fluorescence-labeled OVA-liposome conjugates that contained a final concentration of 4 µg/ml OVA.
Each well contained a final total reaction volume of 25 μl.
Similar(19)
An envelope is delivered to Jake's office containing a final, handwritten will that reverses the first one.
Inflection marks categories such as person, tense, and case; e.g., "sings" contains a final -s, marker of the 3rd person singular, and the German Mannes consists of the stem Mann and the genitive singular inflection -es.
Their cautious reunion, their nervous gladness contains a final split-second in which they look aghast, as if on the edge of the void that may be their married life, for it is a play about the perils of an arranged marriage in which the bride herself has done all the arranging.
This in turn infers that the diluted aliquot should contain a final concentration at this level.
[OM] contains a final summing up of Chisholm's metaphysical system.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com