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This results in one or more MUL-trees, all of which exhibit the same collection of clusters and that contain the backbone tree as a subtree.
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The site contains the backbone atoms of Met26, Val32, and Val34 and side chain of Lys31 (Figure 5G).
In addition, a PyMOL.pse session file showing the regions of interest and an excel file containing the backbone RMSD data computed from the simulated trajectory are made available to download.
The user also gets an excel file containing the backbone RMSD values for K17I and L35P mutants, which can be downloaded and analyzed by the user in order to infer the overall stability of the mutants.
The larger fragment was found to contain the AAV backbone, necessary for plasmid replication in E. coli, and for viral packaging.
Two donor acceptor (D−A) copolymers, PEHBDT-BTz and PODBDT-BTz, containing the same backbone of benzodithiophene (BDT) and bithiazole (BTz) units but different side chains were designed and synthesized.
The digestion yielded a HF-soluble fraction and the HF-insoluble fraction containing the PGN backbone.
The 6.3-kb DNA segment containing the plasmid backbone was extracted from gel and its 3' recessed ends were filled in with Klenow polymerase (Fermentas).
Isolation and restriction with BglI of the plasmids with the American type tetM determinant confirmed that these plasmids indeed contained the plasmid backbone of Dutch type conjugative with the American type tetM determinant integrated (data not shown).
inoculation of 20 μl of IRIV, or 20 μl IRIV containing the plasmid backbone (pcDNA3).
The negative control adenovirus (referred to as NCV, pACCMVpLpAloxD) contains the same backbone as the other constructs.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com