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Existing methods of circRNA expression thus typically involve genes that have been engineered to contain sequence elements that promote back-splicing.
To attach a His6-tag to the C-terminus of MotB or MotS, oligonucleotides that contain sequence encoding a His6-tag at the 3′ end of motB or motS were designed (Table S9).
The deduced peptides revealed that they contain the putative signal peptides and encode for mature peptides, which contain sequence architecture similar to a 6.5-kDa proline-rich AMP of the shore crab, Carcinus maenas which showed similarity with the bactenecin7.
Epitopes presented by protective HLA class I alleles were more likely to elicit a CTL response (P = 0.001) and less likely to contain sequence variation (P = 0.006).
The smallest units of DNA that contain sequence information are the 16 dinucleotides and characterizations of DNA structure at this level have yielded important insights into the factors which affect the conformational properties of DNA and its packaging into nucleosomes.
Throughout our analysis we did not take the expression level into account; rather we assumed that proteins are equally expressed no matter whether they contain sequence variants or not.
With the careful application of error-tolerant search methodologies, such as de novo peptide sequencing, or the USTags approach [8], additional identifications of orthologous proteins that contain sequence polymorphisms may result.
Analysis of internal gene model deviations suggest 42% of the expressed and selected set of L. bicolor gene models contain sequence deviations that largely represent intron/exon boundaries that do not map to the mRNA sequence data (Figure 3).
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The mitochondrial and the ITS data sets contain sequence portions that are highly variable.
An underlying assumption of regular peak finder algorithms is that DNA-binding proteins, such as transcription factors, contain sequence-specific DNA binding domains that target a cluster of cis-acting DNA elements sharing certain sequence features.
To identify regions predicted to contain sequence-defined long-range chromatin structures, Fourier transforms of the period-10 VWG/CWB oscillations in 100 kb windows were computed across all of the human chromosomes except for Y, as described in Materials and Methods.
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